A Simple Protocol for Initial Micropropagation of Musa acuminata cv. Berangan (AAA)
Keywords:
Berangan, banana, plant tissue culture, micropropagation, contamination, aseptic techniqueAbstract
This study presents an observational investigation into the in vitro propagation of Musa acuminata cv. Berangan (AAA), conducted during a 15-week industrial training program at the Plant Biotechnology Incubator Unit (PBIU), Universiti Malaya. Growth progression, contamination rates, and aseptic practices were evaluated during initiation and multiplication stages. Two culture batches, Batch 1 and Batch 2 were established on Murashige and Skoog (MS) basal medium supplemented with 6-benzylaminopurine (BAP), achieving clean culture rates of 89.6% and 98.6%, respectively. The findings highlight contamination sources and emphasize the importance of strict aseptic handling, accurate media preparation, and systematic monitoring to enhance micropropagation efficiency.
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References
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